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FLHGSRAC

Plant Protection

Development of a Duplex IC-RT-qPCR for Large-Scale Detection of Potato Mop-Top Virus in Dormant Potato Tubers

Monday, July 28, 2025
10:30 AM - 10:45 AM CDT
Location: Hall of Ideas EH
Potato mop-top virus (PMTV) infects potato crops causing tuber necrosis which substantially affect the quality of potato tubers rendering them unmarketable. The reliable and large-scale detection method of PMTV, especially in asymptomatic tubers, is a crucial step toward the effective control of PMTV. We developed a duplex Immunocapture Reverse-Transcription Quantitative Polymerase Chain Reaction (IC-RT-qPCR) for the large-scale detection of PMTV. The IC step replaced the laborious and time-consuming RNA extraction step, enabling large scale testing. The duplex format of two primer-probe sets targeting two different RNAs of PMTV genome enhances the reliability of the current assay and reduces the chance of false negatives. Peels from the stem end of the tubers were more likely to test positive for PMTV than bud end peels or lateral tuber cores, which increases the reliability of dormant tuber sampling. The duplex IC-RT-qPCR provides a reliable and sensitive optional tool for the large-scale detection of PMTV in dormant tubers and will help safeguard potato movement in the U.S. and internationally.