Wound healing non-destructive assessment using periderm dry matter and near-infrared hyperspectral imaging (NIR-HSI)

Tuesday, July 29, 2025

9:30 AM - 9:45 AM CDT

Location: Hall of Ideas EH

Presenting Author(s)

HH

Hailey Hampton

PhD Student
University of Idaho
Twin Falls, Idaho

Author(s)

SP

Samuel Paytosh

Research Associate
University of Idaho
Kimberly, Idaho
JS

Jessica Smith

Research Specialist
University of Idaho
KImberly, Idaho
PY

Pankaj Kumar Yadav

Student
University of Idaho
Kimberly, Idaho
TL

Tie Liu

University of Florida, Horticultural Sciences Department
Gainesville, Florida
MM

Marena Manley

Stellenbosch University, Department of Food Science
Stellenbosch, Western Cape, South Africa
PW

Paul Williams

Stellenbosch University, Department of Food Science
Stellenbosch, Western Cape, South Africa
GT

Gustavo H.A. Teixeira

Assistant Professor and Extension Specialist
University of Idaho
Kimberly, Idaho

Rapid wound healing is essential to avoid shrinkage and pathogen infection. Equally important is an accurate determination of when the wound healing process is completed, as keeping potatoes warm can lead to weight loss and decay development. This study evaluated the feasibility of near-infrared hyperspectral imaging (NIR-HSI), coupled with various chemometric approaches, to monitor wound healing progression in potatoes. In the 2024-2025 season, Clearwater Russet potatoes were harvested and immediately treated with i) control (without any treatment), ii) nitric oxide treatment (0.022 g L^-1) for 5 h, and iii) spray application of 2 % (w/v) calcium chloride (CaCl₂). Following this, potatoes were cured at 10 or 12.7 °C and 95 % RH for 14 days. NIR spectral images were acquired using a Specim FX17 camera every two days over a period of 14 days to develop quantitative (dry matter) models. After temperature stabilization (~23°C), Spectral images of the periderm were collected in the diffuse reflectance mode over the range of 900-1700 nm on two opposite positions of each tuber (n= 252, 224 spectral bands; 640 spatial pixels). After image acquisition, the periderm was collected for dry matter (DM) determination. HSI were pre-processed with standard normal variate (SNV) and Savitzky-Golay first derivative. Dry matter values increased significantly from 17.2% on day 0 to 20.0% on day 12, with no significant difference between days 12 and 14 (20.2%). This might indicate that would healing was completed between days 12 and 14. Full cross-validation resulted in an RMSECV of 0.008%, R_c² of 0.73, and validation in an RMSEP of 0.0081%, and R_p² of 0.70. These results demonstrate that NIR-HSI offers a non-destructive method for determining DM content in intact potato tubers and provides a valid tool for monitoring the wound healing process.